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This case study describes the different on-site sanitation facilities within the eThekwini municipality and the sampling procedures for research proposes for each type of facility, developed by the Pollution Research Group. It also describes the main aspects of the laboratory analytical work performed by the PRG for faecal sludge analysis. This includes the administrative work required prior to the analyses, the storage of the samples, the standard and methods for analyses, data interpretation of the experimental results, disposal and the health and safety procedures along the entire process.